Microbes facing tough times

Jennifer sampling soil before the shelters were set up. Here you can see the control (left) and carbon addition (right) plots.

The activities are as follows:

As the climate changes, Michigan is expected to experience more drought. Droughts are periods of low rainfall when water becomes limiting to organisms. This is a challenge for our agricultural food system. Farmers in Michigan will be planting crops into conditions that make it harder for corn, soybean, and wheat to grow and survive.

Scientists are looking into how crop interactions with other organisms may help. Microbes are microscopic organisms that live in soils everywhere. Some microbes can help crops get through time times. These beneficial microbes are called mutualists. They give plants nutrients and water in exchange for carbon from the plant. Microbes use the carbon they get from plants as food. If plants are stressed and don’t have any carbon to give, microbes get carbon from dead plant material in the soil.

Jennifer is a biologist studying the role of microbes in agriculture. She has always been interested in a career that would help people. As a student, Jennifer thought she would have a career in politics. Along the way, she learned that a career in science is a great way to study questions that may lead to solutions for the challenges we are facing today. Jennifer was drawn to the Kellogg Biological Station, where she joined a team of scientists studying the impacts of climate change and drought on agriculture.

Jennifer and other scientists set out to test ways that we can give mutualists in the soil a boost. She thought, perhaps if we were to give microbes more food, they would be less stressed during a drought and would be able to help out crops growing in these stressful conditions.

To test this idea, Jennifer needed to test how well microbes were doing under different carbon and drought conditions. First, she set up treatments in soybean fields to manipulate the amount of carbon in the soil. She set up control plots where she left the soil alone. She also set up carbon treatment plots where dead plant litter was added to the soil to increase the carbon available to microbes.

Next, Jennifer manipulated the availability of water in her plots to test the microbes under stress. To do this, she set up her plots under shelters that kept out rain. The shelters had sprinklers, which were automated to add specific amounts of water to the plots. This design allowed Jennifer to control the watering schedule for each plot. One shelter treatment was a control, where water was added to the plots every week. This is similar to the schedules of local farmers who add water through irrigation. The other shelter treatment was drought, where plots received no water for six weeks. This experiment was replicated 4 times, meaning there were 4 shelters on the control watering schedule and 4 shelters that were under drought conditions.

A view of one of the shelters used in Jennifer’s experiment.

Finally, Jennifer had to measure how the microbes were doing in each treatment. She did this by measuring their enzyme activity. Enzyme activity is a measure of how active the microbes are. The higher the enzyme activity, the happier the microbes are. To measure this, Jennifer collected soil samples from each plot throughout the growing season and took them to the lab to measure enzyme levels in the soil samples. These enzymes are made by microbes when they are active. She then calculated the mean of all her samples for each treatment combination.

Jennifer predicted two things. First, if drought is harmful to microbes, then she would expect to see lower enzyme activity in the drought treatment compared to the irrigated treatment. Second, if adding carbon to the soil is a way to help microbes overcome the challenge of drought, she expected higher enzyme activity in the plots with plant litter added compared to the control treatment. Both of these taken together would indicate that drought is stressful for microbes, but we can help them out by adding resources like plant litter to soils.

Featured scientist: Jennifer Jones (she/her) from the Kellogg Biological Station Long Term Ecological Research Site. Written with Melissa Frost and Liz Schultheis.

Flesch–Kincaid Reading Grade Level = 8.2

Additional teacher resources related to this Data Nugget:

To introduce this Data Nuggets activity, students can watch a talk by Jennifer when she made a classroom visit to share her background and research interests. This video is a great way to introduce students to scientist role models and learn more about what a career in science looks like, as well as get an introduction to the themes in the research.

There is also a video of Jennifer and her scientist colleague, Grant Falvo, out in the field talking about their research under the rainout shelters.

For more information about the rainout shelter experiment, students can watch this short video featuring Jennifer Jones and another scientist on the team, Grant Falvo:

  • https://youtu.be/1lDUQIxwRaM

These data are part of the Kellogg Biological Station Long Term Ecological Research Program (KBS LTER). To learn more about the KBS LTER, visit their website:

  • https://lter.kbs.msu.edu

Do you feel the urban heat?

Attaching a heat sensor to a street sign in Miami to monitor urban temperatures.

The activities are as follows:

Record-breaking temperatures climb higher every year, and Florida is no exception. In Florida, the impact of climate change is felt mostly during the hurricane season. Storms are becoming more violent and show up earlier in the season. These extreme temperatures and weather events affect living organisms of all types, including humans. Outdoor workers, the elderly, and all people who lack adequate housing are susceptible to temperature changes in the environment.

Heat sensor ready to be put out into the city.

Irvin teaches science at a high school in Miami, Florida. On his way to work, he listens to a local radio station to catch up on the news. One day the radio hosts were talking about an increase in homelessness in Miami and other cities. They also brought up the record heat that the U.S. was experiencing and how this may affect those without homes. This conversation on the radio made Irvin think. He reflected on the impact that such high heat could have on individuals who sleep without air conditioning.

This inspired Irvin to learn more about what could be done to mitigate the impact of climate change in his city. Irvin joined a program that invites teachers to work in scientists’ labs in the summer to gain research experience. Irvin was matched with Tiffany, a scientist interested in how urban heat can change based on structures like concrete buildings, urban dwellings, and unshaded places. Irvin took this opportunity to explore how high temperatures in Miami affect the daily lives of people living there. First, Irvin started looking into how temperatures are reported in Miami. He learned that there was just a single sensor stationed at the nearby airport. The heat and humidity readings from this one sensor are used by local officials to alert the entire city about dangerous heat levels. Alerts are issued when the heat index reaches 108 degrees Fahrenheit or higher. Heat index is a value that represents how the body feels temperature when humidity is factored in. With these alerts, people can take action by spending less time outside.

Teachers visiting the mangroves in Miami on a record heat day.

Irvin realized that no matter how reliable the sensor at the airport is, there is likely a larger range of temperatures within the city. He wanted to know whether the temperatures at the airport were similar to the heat felt at places where people spend time outside.

Tiffany’s research team had already started to collect temperature data in urban places where they hadn’t been recorded before. Since 2018, her lab placed hundreds of small heat sensors around the city. The sensors go out for 3 months and then the team collects them, records their data, and places them back out into new areas of the city.

Irvin wanted to compare areas that varied in coverage from the sun. He focused on sites where people gathered and spent long periods of time outside – bus stops. Some of the sites he chose had shade from trees, some had a roof providing partial sun cover, and other sites were totally exposed with no shade. Irvin took photos of each bus stop and used them to classify all sites as either full coverage, partial coverage, or no coverage. He used data from the airport as a control comparison to his bus stop sites.

Featured scientists: Irvin E. Arce (he/him) and Tiffany Troxler (she/her) from Florida International University

Flesch–Kincaid Reading Grade Level = 9.6

Does the heat turn caterpillars into cannibals?

Kale in the lab setting up an experiment with fall armyworms.

The activities are as follows:

Around the world, temperatures are rising from climate change. This is a hot topic for scientists because warmer temperatures could make diseases spread a lot faster. Many diseases spread by the foods we eat. With warmer temperatures, metabolisms increase, and organisms need to eat more food to survive. This increases the risk of eating something that will get them sick.

When Kale started graduate school, they joined a lab that studies how climate change affects the spread of disease in fall armyworms, a type of caterpillar. Fall armyworms are an agricultural pest known for destroying corn, soybeans, and other crops worldwide. In the summer, they move into fields and rapidly chow down on crops. It’s often reported by farmers that it seems as though fall armyworms can remove all the leaves from a cornfield overnight! Believe it or not, their huge appetite leads them to another food source – they will even turn into cannibals and eat each other!

Once Kale started graduate school, they became interested in how cannibalism can increase disease spread in warmer temperatures. Fall armyworms can get infected with a special type of virus called a baculovirus. Baculoviruses are a group of viruses that infect insects, especially caterpillars. They are highly specialized, meaning that each baculovirus usually only infects one species.

A fall armyworm that has been liquified due to a baculovirus infection.

If a fall armyworm eats a fellow fall armyworm that is infected, it can be deadly. In fact, the disease causes their body to completely liquify into a puddle of pure virus! This baculovirus is so effective that farmers even use it to help control infestations in their fields. Since this specific baculovirus only infects fall armyworms, it is safe to use on crops without worrying about effects on humans or other living things.

To study how cannibalism can affect disease spread, Kale designed a set of experiments. They thought that when temperatures are higher, the larvae’s metabolism would increase and make them hungrier caterpillars. Increased appetite could then lead to more cannibalism. As a result, more larvae would be eating others that are infected, further spreading the deadly baculovirus.

To test these ideas, Kale set up small Petri dishes and placed one big fall armyworm in each dish as the focus of each trial. Kale added a piece of insect food and a smaller fall armyworm to each dish. This way, the larger caterpillars had the option of eating the insect food, cannibalizing its smaller friend, or munching on both.

To see if temperature had an impact, Kale set up three treatments at low, medium (ideal), and high temperatures. They assigned 40 Petri dishes to each temperature. To test changes in disease transmission, half of the smaller caterpillars were infected with baculovirus, and half remained uninfected.

Kale predicted that fall armyworms at higher temperatures would cannibalize more because they need more food to keep up with an increased metabolism. They also predicted that fall armyworms that eat an infected caterpillar would be more likely to become infected at higher temperatures.

Featured scientists: Kale Rougeau from Louisiana State University

Flesch–Kincaid Reading Grade Level = 10.2

Additional teacher resources related to this Data Nugget include:

You can also watch a time-lapse video of Kale in the lab to get a glimpse of their work. Follow along as they check fall armyworm cadaver samples for baculovirus infection using a microscope
Kale also provided a video of baculovirus lysing, where occlusion bodies that encapsulate the virus are dissolved, confirming the presence of infection in the fall armyworm sample.
  • Read more about Kale’s hobby of participating and training for dog competitions on the Beyond the Bench blog.
  • More about fall armyworms here and here.

Too hot to help? Friendship in a changing climate

This coral has lost its algae partners, causing it to be bleached. (Photo by Coffroth Lab)

The activities are as follows:

When given emergency instructions on a flight, you’re told to put on your own oxygen mask before assisting others. This is because if you run out of oxygen, you won’t be able to help others. Turning to nature, this same idea may be true when we look at relationships between two species.

Coral and certain types of algae form a mutualism where both species benefit from the partnership. Coral provides a safe home for algae, and algae make food for coral through photosynthesis. However, climate change is causing warmer ocean temperatures that stress the relationship. If the water gets too hot for algae, they can’t make food for the coral anymore. To survive, the algae must help themselves before they can help the coral.

Casey is a biologist interested in studying the changing coral-algae mutualism. He wants to know whether different individuals of the same algae species do better than others in warming waters. Individuals of the same species can have different traits. For example, each human person belongs to the same species, but each of us has different traits. This is largely because of our genetic composition for these traits, or genotypes. Casey set out to test if different algae genotypes were capable of being better mutualists under warm temperatures. If he could identify these genotypes, then maybe that could help protect coral in the future.

Casey gets a sample of algae from a flask in his lab. (Photo by David J. Hawkins)

Casey and his graduate student, Richard, set up experiments to test algae genotypes to see how well they performed at different temperatures. Casey and Richard grew five different genotypes of the same algae species in the lab. They used a pipette to transfer 10,000 cells of each genotype and placed them in flasks at two different temperatures. The lower temperature treatment is one where corals and their algae are usually happy: 26 degrees Celsius. The higher temperature treatment is where coral’s relationship with algae starts to break down: 30 degrees Celsius. At that temperature, many corals lose their algae entirely, in a process called coral bleaching.

Casey and Richard measured two things – the total amount of photosynthesis and the total amount of respiration happening in each flask. They did this by tracking what happened to oxygen over time. When there is a lot of photosynthesis, oxygen goes up, and when there is a lot of respiration, oxygen goes down. Two conditions are best for the mutualism. First, a lot of photosynthesis means the algae produced more food that they can share with coral. Second, less respiration means the algae used less of the food for themselves and have more to share with the coral. In summary, when the algae is stressed it does less photosynthesis and more respiration, making it a worse trading partner for coral. The best algae partner is the genotype that can photosynthesize the most and respire the least. The net food available is how much of the food made through photosynthesis is available after subtracting the food used by respiration.

Featured scientists: Casey terHorst (he/him) and Richard Rachman (he/him)

from California State University Northridge

Flesch–Kincaid Reading Grade Level = 8.9

Additional teacher resources related to this Data Nugget

Do urchins flip out in hot water?

Erin in the urchin lab at UC-Santa Barbara.

The Reading Level 1 activities are as follows:

The Reading Level 3 activities are as follows:

Teacher Resources:

Imagine you are a sea urchin. You’re a marine animal that attaches to hard surfaces for stability. You are covered in spikes to protect you from predators. You eat giant kelp – a type of seaweed. You prefer temperate water, typically between 5 to 16°C. But you’ve noticed that some days the ocean around you feels too hot. 

These periods of unusual warming in the ocean are called marine heatwaves. During marine heatwaves, water gets 2-3 degrees hotter than normal. That might not sound like much, but for an urchin, it is a lot. The ocean’s temperature is normally very consistent, so urchins are used to a small range of temperatures. Urchins are cold-blooded. This means they can’t control their own body temperature and rely on the water around them. Whatever temperature the ocean water is, they are too!

Erin is a scientist who studies how environmental changes, like temperature, affect organisms. Erin first got excited about urchins when she interned with a research lab. When she started graduate school, she learned more about their biology and started to ask questions about how urchins would react to marine heatwaves. Hot water can speed up animals’ metabolisms, making them move and eat more. However, warmer temperatures can also cause stress, potentially causing urchins to be clumsier and confused.

Erin getting ready to scuba dive to look for urchins off the California coast.

One summer, two science teachers, Emily and Traci, came to California to work in the same lab as Erin. Emily and Traci wanted to do science research so they can share their experience with their students.  As a team, they decided to test whether marine heat waves could be stressing urchins by looking at a simple behavior that they could easily measure. Healthy urchins have a righting instinct to flip over to orient themselves “the right way” using their sticky tube feet.

The research team predicted that urchins would be slower to right themselves in warmer temperatures. However, they also thought the response could depend on the temperature the urchins were used to living in. If the urchins had been acclimated to higher temperatures, they might not be as strongly affected by the heatwaves.

Together, Erin, Emily, and Traci took 20 urchins into her lab and split them into 2 groups. Ten were kept at 15°C, the ocean’s normal temperature in summer. The other ten were kept at 18°C, a marine heatwave temperature. They let the urchins acclimate to these temperatures for 2 weeks. They tested how long it took each urchin to right itself after being flipped over. They did this at three temperatures for each urchin: 15°C (normal ocean), 18°C (heatwave), and 21°C (extreme heatwave). They worked together to test the urchins three times at each temperature to get three replicates. Then they calculated the average of each urchin’s responses.

Featured scientists: Erin de Leon Sanchez (she/her) from University of California – Santa Barbara, Emily Chittick (she/her), and Traci Kennedy (she/her) from Milwaukee Public Schools.

Flesch–Kincaid Reading Grade Level = The Content Level 3 activity has a score of 7.9 ; the Level 1 has a score of 5.9

Additional teacher resources related to this Data Nugget include:

  • Here is a video of a parrotfish finding and eating an urchin. Show this video to emphasize how important it is for urchins to be able to right themselves!
Video of a trial where the researchers flipped over an urchin and timed how long it took the urchin to flip back over.
Watch how sea urchins use items from their environment to cover themselves.

Poop, poop, goose!

Cackling Goose next to a pile of goose poop, or feces
Cackling Goose next to a pile of goose poop, or feces. Photo by Andrea Pokrzywinski.

The activities are as follows:

Each spring, millions of birds return to the Yukon-Kuskokwim Delta. This delta is where two of the largest rivers in Alaska empty into the Bering Sea. It is also one of the world’s most significant habitats for geese to breed and raise their young. 

With all these geese coming together in one area, they create quite a mess – they drop tons of poop onto the soil. So much poop in fact, that scientists wonder whether poop from this area in Alaska could have a global impact! Climate change is a worldwide environmental issue that is caused by too many greenhouse gasses being released into our atmosphere. Typically, we think of humans as the cause of this greenhouse gas release, but other animals can contribute as well. 

When poop falls onto the soil it is decomposed by bacteria. Bacteria release methane (CH4), a potent greenhouse gas. The more geese there are, the more poop they will produce and the more food there will be for soil bacteria. By increasing the amount of greenhouse gasses that are released by soil bacteria, geese might actually indirectly contribute to global climate change.

Trisha is an ecosystem ecologist who scoops goose poop for research projects. Her research is looking into whether animals, other than humans, can change the carbon cycle. Trisha teamed up with Bonnie, a fellow ecosystem ecologist. Bonnie studies how matter moves between the living parts of the environment, such as plants and animals, and the nonliving parts. She is especially interested in how bacteria in the soil play a role in the carbon cycle.

Together, the team designed a three-year project to figure out the effects of goose poop on the carbon cycle. Each summer, a large team of researchers spend 90 days camping on remote sites near the Yukon-Kuskokwim Delta. The team scooped up poop from nearby goose habitats to use in their experiments. They set up six control plots where they added no poop and six treatment plots where they added poop. From these twelve plots, the team measured methane emissions from the soil. Methane was measured as methane flux in micromoles, or µM. These data helped them determine how ecosystems respond to geese by measuring whether goose poop affects methane production by soil bacteria.  

Featured scientists: Trisha Atwood of Utah State University and Bonnie Waring of Imperial College. Written by Andrea Pokrzywinski.

Flesch–Kincaid Reading Grade Level = 8.7

Additional teacher resources related to this Data Nugget include:

Sink or source? How grazing geese impact the carbon cycle

Tricia (left) installing carbon dioxide plots in the field.

The activities are as follows:

“If it wasn’t for the geese, you and I would not be here today because our ancestors would not have made it. When long, hard winters emptied people’s food caches early, starvation loomed. Return of geese in April saved us.” – Chuck Hunt, born and raised on the Yukon-Kuskokwim Delta

Spring geese are an essential food source for subsistence communities like Chevak, Alaska. Elders in western Alaska Native communities have observed a decrease in geese returning to their villages over time. These changes affect the local communities and could also affect the local ecosystem.

One way geese change their environment is by eating grass. In the Yukon-Kuskokwim Delta in western Alaska, birds from every continent on Earth migrate to this sub-Arctic habitat to lay their eggs and raise their young. Once they arrive, geese eat a ton of grass. They graze only in specific areas, called grazing lawns, leaving the rest of the vegetation alone.

When geese graze on wetland plants, they remove plant matter, potentially decreasing the amount of carbon dioxide, or CO2, that is released during photosynthesis. As plants photosynthesize, they absorb CO2 from the atmosphere and turn it into glucose (a sugar) and oxygen. Gross primary production is the total amount of energy that plants capture from sunlight to grow and live before they use up some of that energy for themselves. Plants can slow climate change by removing CO2 from the atmosphere and turning it into plant matter, like leaves and roots.

A scientist mimics geese grazing by clipping the grass.

Trisha is a scientist who became interested in ways that animals can affect the carbon cycle through their interactions with the environment. She wondered whether fewer geese returning to western Alaska could have global consequences that extend beyond remote communities. She thought that if geese ate enough grass, they may limit photosynthesis. This is important because it could change whether this ecosystem is a carbon sink or a carbon source. An ecosystem is called a carbon sink if it absorbs more CO2 through photosynthesis than it releases through respiration. Alternatively, an ecosystem can be a carbon source if more CO2 is released than absorbed. We want ecosystems to be carbon sinks because then they keep CO2 out of the atmosphere, where it contributes to global warming.

To test her idea, Trisha teamed up with fellow scientists Bonnie, Karen, and Jaron to take a closer look at how grazing grass influences whether the Y-K Delta ecosystem is releasing or absorbing CO2. To do their experiment they had to get creative. They considered getting a lot of geese, bringing them to an ungrazed area, and letting them chow down. However, it’s hard to capture geese and get them to graze exactly where you want. So instead, the research team simulated the effects of geese by cutting the grass to mimic nibbling and then gently vacuuming the pieces of grass to remove them.

The “Carbon and Geese” scientist team.

The team set up six different experimental areas. Inside each area were two plots: one that was left ungrazed, and the other which was artificially grazed. The research team then used a piece of equipment called a LI-COR to measure the quantity of CO2 in the air above each plot. They recorded the CO2 levels during the day and night. The comparison from day to night is one way to look at gross primary production and respiration in a system. At night, when there is no light, plants can’t photosynthesize, so the detected CO2 will be from respiration. The levels during the day represent a combination of CO2 absorption by plants and release from respiration.

To assess whether the ecosystem is a carbon sink or source, we need to determine the difference between respiration and gross primary production, or net ecosystem exchange (NEE). A negative NEE means the ecosystem absorbs more CO2 than it emits. A positive NEE means the ecosystem is releasing more CO2 than it is absorbing. In this way, scientists classify an ecosystem as either a carbon sink that is storing carbon or a carbon source that is releasing carbon into the atmosphere.

Featured scientists: Trisha Atwood, Karen Beard, and Jaron Adkins from Utah State University and Bonnie Waring from Imperial College. Written by Andrea Pokrzywinski.

Flesch–Kincaid Reading Grade Level: 8.9

Additional teacher resources related to this Data Nugget:

Check out this website created by teacher Andrea who participated in the research and wrote this Data Nugget. You will find additional lesson plans, videos, slides, and articles to use in the classroom!

Seagrass survival in a super salty lagoon

A researcher in the Dunton Lab measures seagrasses underwater using a mask, snorkel, and a white PVC quadrat.

The activities are as follows:

Seagrasses are a group of plants that can live completely submerged underwater. They grow in the salty waters along coastal areas. Seagrasses are important because they provide a lot of benefits for other species. Like land plants, seagrasses use sunlight and carbon dioxide to grow and produce oxygen in a process called photosynthesis. The oxygen is then used by other organisms, such as animals, for respiration. Other organisms use seagrasses for food and habitat. Seagrass roots hold sediments in place, creating a more stable ocean bottom. In addition, the presence of seagrasses in coastal areas slows down waves and absorbs some of the energy, protecting shorelines.

Unfortunately, seagrasses are disappearing worldwide. Some reasons include damage from boats, disease, environmental changes, and storms. Seagrasses are sensitive to changes in their environment because they have particular conditions that they prefer. Temperature and light levels control how fast the plants can grow while salinity levels can limit their growth. Therefore, it is important to understand how these conditions are changing so that we can predict how seagrass communities might change as well.

Ken is a plant ecologist who has been monitoring seagrasses in southern Texas for over 30 years! Because of his long-term monitoring of the seagrasses in this area, Ken noticed that some seagrass species seemed to be in decline. Kyle started working with Ken during graduate school and wanted to understand more about what environmental conditions might have caused these changes. 

Manatee grass (Syringodium filiforme) located within the Upper Laguna Madre.

Texas has more seagrasses than almost any other state, and most of these plants are found in a place called Laguna Madre. During his yearly seagrass monitoring, Ken noticed that from 2012 – 2014 one of the common seagrasses, called manatee grass, died at many locations across Laguna Madre. Since then, the seagrass has grown back in some places, but not others. Kyle thought this would be an opportunity to look back at the long-term dataset that Ken has been collecting to see if there are any trends in environmental conditions in years with seagrass declines.

Each year, Ken, Kyle, and other scientists follow the same research protocols to collect data to monitor Laguna Madre meadows. Seagrass sampling takes place 2 – 4 times a year, even in winter! To find the manatee grass density, scientists dig out a 78.5 cm2 circular section (10 cm diameter) of the seagrass bed while snorkeling. They then bring samples back to the lab and count the number of seagrasses. While they are in the field, they also measure environmental conditions, like water temperature and salinity. A sensor is left in the meadow that continuously measures the amount of light that reaches the depth of the seagrass.

Kyle used data from this long-term monitoring to investigate his question about how environmental conditions may have impacted manatee grass. For each variable, he calculated the average across the sampling dates to obtain one value for that year. He wanted to compare manatee grass density with salinity, water temperature, and light levels that reach manatee grass. He thought there could be trends in environmental conditions in the years that manatee grass had low or high densities.

Featured scientists: Kyle Capistrant-Fossa (he/him) & Ken Dunton (he/him) from the U-Texas at Austin

Flesch–Kincaid Reading Grade Level 9.8

Additional teacher resources related to this Data Nugget:

There is another Data Nugget that looks at these seagrass meadows! Follow Megan and Kevin as they look at how photosynthesis can be monitored through the sound of bubbles and the acoustic data they produce.

Follow this link for more information on the Texas Seagrass Monitoring Program, including additional datasets to examine with students.

There are articles in peer-reviewed scientific journals related to this research, including:

National Park Service information about the Gulf Coast Inventory and Monitoring.

Texas Parks and Wildlife information on seagrass:

A difficult drought

A field of switchgrass studied by biofuels researchers.

The activities are as follows:

Most people use fossil fuels like natural gas, coal, and oil daily. We use them to generate much of the energy that gets us from place to place, power our homes, and more. Fossil fuels are very efficient at producing energy, but they also come with negative consequences. For example, when burned, they release greenhouse gases like carbon dioxide into our atmosphere. The right balance of greenhouse gasses is needed to keep our planet warm enough to live on. However, because we have burned so many fossil fuels, the earth has gotten too hot too fast, resulting in climate change. Scientists are looking for other ways to fuel our lives with less damage to our environment.

Substituting fossil fuels with biofuels is one of these options. Biofuels are fuels made from plants. Unlike fossil fuels, which take millions of years to form, biofuels are renewable. They are made from plants grown and harvested every few years. Using biofuels instead of fossil fuels can be better for our environment because they do not release ancient carbon like burning fossil fuels does. In addition, the plants made into biofuels take in carbon dioxide from the atmosphere as they grow.

To become biofuels, plants need to go through a series of chemical and physical processes. The sugar stored in plant cells must undergo fermentation. In this process, microorganisms, like yeast, transform the sugars into ethanol that can be used for fuels. Trey is a scientist at the Great Lakes Bioenergy Center. He is interested in seeing how yeast’s ability to transform sugar into fuel is affected by environmental conditions in fields, such as temperature and rainfall.

When there was a major drought in 2012, Trey used the opportunity to study the impacts of drought. The growing season had very high temperatures and very low rainfall. These conditions make it more difficult for plants to grow, including switchgrass, a prairie grass being studied as a potential biofuel source.

Trey knew that drought affects the amount and quality of switchgrass that can be harvested. He wanted to find out if drought also had effects on the ability of yeast to transform the plants’ sugars into ethanol. Stress from droughts is known to cause a build-up of compounds in plant cells that help them survive during drought. Trey thought that these extra compounds might harm the yeast or make it difficult for the yeast to break down the sugars during the fermentation process. Trey and his team predicted that if they fed yeast a sample of switchgrass grown during the 2012 drought, the yeast would struggle to ferment its sugars and produce fewer biofuels as a result.  

To test their idea, the team studied two different sets of switchgrass samples that were grown and collected in Wisconsin. One set of switchgrass was grown in 2010 under normal conditions. The other set was grown during the 2012 drought. The team introduced the two samples to yeast in a controlled setting and performed four fermentation tests for each set of switchgrass. They recorded the amount of ethanol produced during each test.

Featured scientists: Trey Sato from the University of Wisconsin-Madison. Written by Marina Kerekes.

Flesch–Kincaid Reading Grade Level = 8.2

Additional teacher resources related to this Data Nugget include:

There are other Data Nuggets that share biofuels research. Search this table for “GLBRC” to find more! Some of the popular activities include:

The Great Lakes Bioenergy Research Center (GLBRC) has many biofuel-related resources available to K16 educators on their webpage.

For activities related specifically to this Data Nugget, see:

Changing climates in the Rocky Mountains

Lower elevation site in the Rocky Mountains: Temperate conifer forest. Photo Credit: Alice Stears.

The activities are as follows:

Each type of plant needs specific conditions to grow and thrive. If conditions change, such as temperature or the amount of precipitation, plant communities may change as well. For example, as the climate warms, plant species might start to shift to higher latitudes to follow the conditions where they grow best. But what if a species does well in cold climates found at the tops of mountains? Because they have nowhere to go, warming puts that plant species at risk.  

To figure out if species are moving, we need to know where they’ve lived in the past, and if climates are changing. One way that we can study both things is to use the Global Vegetation Project. The goal of this project is to curate a global database of plant photos that can be used by educators and students around the world. Any individual can upload photos and identify plant species. The project then connects each photo to information on the location’s biome, ecoregion, and climate, including data tracking precipitation and temperature over time. The platform can also be used to explore how the climates of different regions are changing and use that information to predict how plant communities may change. 

Daniel is a scientist who is interested in sharing the Global Vegetation Project data with students. Daniel became interested in plants and vegetation when he learned in college that you can simply walk through the woods and prairie, collect wild seeds, germinate the plants, and grow them to restore degraded landscapes. Plants set the backdrop for virtually every landscape that we see. He thinks plants deserve our undivided attention.

Daniel and his team wanted to create a resource where students can look deeper into plant communities and their climates. Much of the inspiration for the Global Vegetation Project came from the limitations to undergraduate field research during the COVID-19 pandemic. Students in ecology and botany classes, who would normally observe and study plants in the field, were prevented from having these opportunities. By building an online database with photos of plants, students can explore local plants without having to go into the field and can even see plants from faraway places. 

Daniel’s lab is based in the Rocky Mountains in Wyoming, where the plants are a showcase in both biodiversity and beauty. These communities deal with harsh conditions: cold, windy and snowy winters, hot and dry summers, and unpredictable weather during spring and fall. The plants rely on winter snow slowly melting over spring and into summer, providing moisture that can help them survive the dry summers. 

The Rocky Mountains are currently facing many changes due to climate change, including drought, increased summer temperatures, wildfires, and more. This creates additional challenges for the plants of the Rockies. Drought reduces the amount of precipitation, decreasing the amount of water available to plants. In addition, warmer temperatures in winter and spring shift more precipitation to rain instead of snow and melts snow more quickly. Rain and melted snow rapidly move through the landscape, becoming less available to plants in need. On top of all this, hotter, drier summers further decrease the amount of water available, stressing plants in an already harsh environment. If these trends continue, there could be significant impact on the types of plants that are able to grow in the Rocky Mountains. These changes will have an impact on the landscape, organisms that rely on plants, and humans as well.

Daniel and his colleagues pulled climate data from a Historic period (1961-2009) and Current period (2010-2018). They selected two locations in Wyoming to focus on: a lower elevation montane forest and a higher elevation site. To study climate, they focused on temperature and precipitation because they are important for plants. They wanted to study how temperature and precipitation patterns changed overall and how they changed in different seasons. They predicated temperatures would be higher in the Current period compared to the Historic period in both locations. For precipitation, they predicted there would be drier summers and wetter springs.

Featured scientist: Daniel Laughlin from The University of Wyoming. Written by: Matt Bisk.

Flesch–Kincaid Reading Grade Level = 10.5

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